This play mode is located in the main menu under Training.
Before searching for a match, the party leader must select a difficulty: Easy, Medium, or Hard. Higher difficulties improve accuracy and damage output of the AI. Easy deals 25% damage, Medium 50%, and Hard 100%. Bots cannot deal critical damage through headshots.
Only a handful of heroes are programmed into the game as bots. The heroes which can be encountered on the AI team are as follows: Roadhog, Zarya, Bastion, Cassidy, Mei, Reaper, Soldier: 76, Sombra, Torbjörn, Ana, Lúcio, and Zenyatta.
Except for special events, Practice vs. AI is the sole mode with matchmaking that contains bots.
Practice vs. AI always yields 90% experience, regardless of difficulty.